Jm109 ampicillin

Susceptible and resistantstrains of Escherichia coli JM109 to ampicillin and those of Staphylococcus epidermidis to tetracycline, served as a blind test, were examined.. the use in the United States expired on December 2014. This patent protected also BL21 (DE3), BLR (DE3), HMS174 (DE3), JM109 (DE3), and OrigamiB(DE3).BIO VIEW TAKARA BIO EUROPE. reactant was applied to E. coli JM109 competent cells (7.3×108 cfu/µg pUC118 DNA) for transformation. Transformed bacteria were.A multiple antibiotic and serum resistant oligotrophic strain, Klebsiella pneumoniaeMB45 having novel dfrA30, is sensitive to ZnO QDs.FAQ about JM109 cells from Promega Corporation. Recherche & Développement de molécules cibles.Not for use in diagnostic procedures for clinical purposes. For IN VITRO USE ONLY. ISO 9001/14001 Certified Company MacCellTM 9– JM109 10. with ampicillin.H-3 HIT-JM109. into HIT Competent Cells • LacZ Complementation for Blue/White screening • Ampicillin marker for antibiotic.

. ampicillin or tetracycline. JM101 and JM109 (suitable for growing M13 phages), XL1-Blue (general-purpose, good for blue/white lacZ screening).

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Combinatorial mutagenesis to restrict amino acid. genomic DNA of E. coli JM109 by the PCR method using a pair of. and histidine, and either 100 g ml 1 ampicillin or.a AMP, ampicillin; CHL, chloramphenicol; STR, streptomycin; SMX,. E. coli JM109 was used as a recipient strain. The cloned products were purified using the.

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E. coli strain JM109 and DNA of selected recombinant clones was. containing 100 lg}ml ampicillin, a 1:50 dilution was grown until the OD ’!! reached 0–6–1.A Complete collection of Competent Cells - shop for chemically competent and electrocompetent cells by strain, application, format, transformation efficiency. https.The pGEM®-T and pGEM®-T Easy Vector Systems now include a 2X Rapid. • 1.2ml JM109 Competent Cells, High Efficiency (6 x 200µl) • 1 Protocol.

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Accueil > Revues > European Cytokine Network > Human interleukin-6. The recombinant protein was produced by expression of the DNA fragment in E. coli strain JM109,.(54) peptide and nucleotide sequences of anisakis spp. and uses thereof. peptid- und nukleotidsequenzen von anisakis spp. und ihre verwendung. sequences peptidiques.

Transformed E. coli JM109 as a biosensor for penicillin Auteur(s) / Author(s) RAVI KIRAN B.; KALE K. U.; Résumé / Abstract.JM109 Host strain for DNA manipulation Sambrook et al. (1989) S17-1 C600<RP-4 2-(Tc<Mu)(Kan<Tn7). Ap r, ampicillin resistant; Cb, carbenicillin resistant.

The ampicillin-resistant plasmid, pI0720, contained the cumene dioxygenase genes and the dimethyl sulfide monooxygenase genes,. In the case of E. coli JM109.. (see Materials and Methods). The GST and GST-Dp71f proteins fusions were isolated by incubation of JM109 bacterial lysates with glutathione-Sepharose beads,.

. and expressed into Escherichia coli JM109. Recombinant enzyme catalyzed oligosaccharides synthesis from sucrose as donor and maltose acceptor.

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phytoplasmas detected in two leafhopper species associated with alfalfa plants infected. recombinant plasmids were inserted into JM109. ed with ampicillin,.Do you have evidence that the transformation of E. coli JM109 with ligated plasmid (either P, or +, or without insert) was successful (or that it was not)?.

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like TOP10, DH5α, JM109, or equivalent. Select transformants on LB agar plates containing 50–100 μg/ml ampicillin. Be sure to prepare a glycerol stock of each.

. (JM109; Promega) using the pGEM-T Easy vector system I (Promega) by following the manufac-turer’sinstructions,and10clonesforeachgeneweresequencedusingan.

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